Abstract:

Canine Parvovirus-2 causes hemorrhagic gastroenteritis in dogs and spreads rapidly in dog population. The virus sheds in large numbers in the feces and thus, feces serve as a source of infection. The disease condition has been complicated further due to emergence of variants over the years. The present study was conducted to know whether the antigenic types of CPV cross protect each other, cross neutralization assay was performed for vaccine strain (CPV 2) and other strains of virus CPV 2a and CPV 2b. Hyperimmune serum was successfully raised in rabbits for the vaccine strain and confirmed by Indirect ELISA. TCID50 was found to be 102.49/ml for CPV 2. Cross-neutralization assay was carried out using 100 TCID50 of virus types and two fold serial dilution of hyperimmune serum in various combinations of virus and serum types. The maximum dilution at which serum against CPV 2 type could neutralize CPV 2a and CPV 2b virus was 1:2048 and the dilution at which serum against CPV 2a could neutralize CPV 2 virus type was 1:4096. Similarly, the maximum dilution at which serum against CPV 2b could neutralize CPV 2 virus type was 1:4096. The titre of the serum CPV 2 for the homologous virus type is higher i.e. 8192 as compared to heterologous virus type.