Effect of Synthetic Auxin on Water Content of Seedlings of Prosopis julifloraSwarz DC

Eltayeb A. H. Suliman¹ , , Sayda O. Elhewairis¹ , Salah E. Elamin²

¹Department of Botany and Agricultural Biotechnology, Faculty of Agriculture, University of Khartoum, Sudan

²Department of Crop Protection, Faculty of Agriculture, University of Khartoum, Sudan

Corresponding Author Email: eltayeb71_sha@yahoo.com

DOI : https://doi.org/10.51470/ABF.2025.4.1.08

Abstract

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. Introduction

The synthetic auxin 2,4-dichlorophenoxy acetic acid (2,4-D) mimics the effects of the natural auxin indole-3-acetic acid (IAA) in plants[1]. Unlike IAA quickly inactivated; 2,4-D persists for extended periods. This prolonged presence can create an imbalance in auxin levels and disrupt interactions with other hormones at the tissue level[2]. 2,4-D accumulates in chloroplasts and is metabolized within these organelles. At low doses, 2,4-D can promote plant growth. However, at high doses, it leads to overgrowth characterized by cupping and stunting leaves, brittleness, stunting and twisting of stems, and overall abnormal growth. High concentrations of 2,4-D inducted the enzyme aminocyclopropane-1-carboxylic acid (ACCS), which increased ethylene biosynthesis. This is followed by an accumulation of abscisic acid (ABA)[3]. The effects of high 2,4-D concentrations include abnormal growth patterns, reductions in stomata aperture, and overproduction of reactive oxygen species (ROS). These changes can lead to chloroplast damage, progressive chlorosis, and the destruction or blockage of the membrane and vascular system in the stem[3].

2,4-D is actively transported into plant cells via a common carrier protein. Metabolism of 2,4-D in plants occurs through direct conjugation and ring hydroxylation. In sensitive dicots, 2,4-D can bind to the TIR1, which leads to the expression of auxin-responsive genes. However, the ring structure of 2,4-D is not hydroxylase by metabolic enzymes. The direct conjugation of 2,4-D with amino acids or glucose results in phytotoxic metabolites that can be hydrolyzed back to 2,4-D[4]. In intolerant monocots, the ring hydroxylation of 2,4-D leads to a non- or partially phytotoxic metabolite[5]. The objective of this research; is to study the effect of 2,4-D on the water content of mesquite seedlings raised from seeds collected from 2,4-D- treated trees.

2. Materials and Methods

2-1. Plant Materials, chemical solutions, and application method

An experiment was conducted in about four hectares, divided into three replicates. In each replicate, thirty mesquite trees were carefully selected and classified into three sizes small, medium, and large. Each size group consisted of ten trees categorized based on tree height and the number of branches per tree. The experiment took place during the flowering stage of the mesquite trees. Before herbicide application, ten inflorescences were chosen and marked on each tree. The herbicide 2,4 -D was applied at four different weights 6, 12, 18, and 24 grams (a.i.), each weight was dissolved in either one liter of water or diesel. Additionally, water and diesel were used as individual controls for comparison. The mixture was applied as a directed spray to the base of the stem, approximately 20-30 cm above ground level on each of the selected trees [6].

2-2. Pods collection, seeds prepared, planting, and data collection

The ripe pods from the marked inflorescences were collected from each treatment within sixty days of application. The seeds were extracted from pods using a Sharpe knife and scissors. Seed dormancy was broken by soaking it in 98% sulfuric acid for a few minutes, and in distilled water for 24 hours. Twenty seeds from each treatment were planted in plastic bags, each measuring 40 cm. in diameter, and equipped with holes in the bottom. The bags were filled with a 5 kg mixture of loam and sand in a 1:1 volume ratio. These plastic bags were kept under temperature and day-length conditions, and seeds were irrigated every two days.

One month after planting, the seedlings from each treatment were removed from the bag and separated into stems and roots. The Mean fresh weight of both was calculated. The shoot and root were dried in the air for three days, and in an oven at 70 C, until the constant weights were achieved. and the water content for both parts was calculated [7].

Fresh weight – dry weight  × 100

fresh weight

2-3. Experimental design, and data analysis

The experiments were arranged as a Factorial Randomized Complete Block Design (FRCBD) with three replications for each treatment. Data were analyzed using analysis of variance (ANOVA). Means were compared for significance with the least significance difference (LSD) test at a significance level of 0,05.

3. Results and Dissuasion

All weights of 2,4-D tested achieved significant differences in shoots and roots water content compared to the control group. The interactions of the weights of 12, 18, and 24 grams a.i. of 2,4-D with diesel solvent resulted in notable differences in shoot water content compared to the control. All tested weights of 2,4-D when combined with diesel or water significantly reduced root water content relative to the control (Table 1).

Diesel solvent significantly decreased shoot water content compared to water solvent. However, no significant difference in root water content between the two solvents. Additionally, there were no significant differences in shoots and root water content among the seedlings from seeds collected from trees of different sizes, small, medium, and large. The interaction between tree sizes and diesel solvent resulted in a significant decrease in shoot water content compared to the water solvent, but root water content showed no significant differences across the interaction of tree sizes and the two solvents (Table 2).

The interaction between the application of 24 grams a.i. of 2,4-D and the three tree sizes resulted in significant differences in shoot water content compared to the control. The seedlings from the three tree sizes were treated with either 18 grams or 24 grams a.i. of 2,4-D dissolved in diesel or 24 grams a.i. Dissolvedin water exhibited a significant decrease in shoot water content compared to the control. There were no significant differences in shoot water content of seedlings raised from the seeds collected from medium and large trees treated with 6, 12, and 18 grams a.i. of 2,4-D dissolved either in diesel or water compared to the control (Table 3).

The interaction between various amounts of 2,4-D and the three tree sizes resulted in significant differences in root water content compared to the control. The root water content of seedlings grown from seeds collected from the three tree sizes and treated with 18 and 24 grams a.i. of 2,4-D dissolved in either diesel or water was significantly lower than that of the control group (Table 4).

The results in the present study indicated that; the use of the herbicide 2,4-D during the flowering stage of mesquite trees led to a decrease in the shoot and root water content of seedlings as the concentration of 2,4-D increased. These findings are consistent with those of [8] who reported that; the use of 2,4-D as a soil application at 1000 g a. i. ha^– applied at various times before sowing soybeans decreased both the fresh and dry weight of shoots and roots. Similarly, [9] found that applying 2,4-D at five different rates (1-5 ppm) resulted in a reduction in the fresh and dry weight of Abelmoschusesculentusafter five days of treatment Additionally, Cassia toraalso treated with different rates of 2,4-D,resulted in a decrease in the dry and fresh weight of both shoots and roots, correlating with increased concentration of the herbicide [10].

In the mesquite stem (dicotyledoneae) the vascular tissues are arranged in rings and contain a cambium. In this case [11] reported that synthetic auxins are not metabolized as quickly as the endogenous auxin indole-3-acetic acid IAA. In contrast, monocotyledoneae stems have vascular tissues, that are scattered in bundles and lack a vascular cambium, in these stems, synthetic auxins can rapidly inactivate through conjugation. Mesquite pods ripen 60 days after flowering[12]. This extended period allows for the absorption of 2,4-D into the seed coat, increasing its concentration within the seeds, and affecting them as a pre-emergence herbicide. At high concentrations, 2,4-D acts as an herbicide and remains persistent in the plant for, an extended time [2]. This led to an overproduction of ethylene, which stimulates the production of hydrogen peroxide (H2O2) [13]. Excess H2O2 can cause oxidative damage resulting in reduced plant biomass production [14] and the destruction of xylem tissues [3]. Additionally, alkanes and polycyclic aromatic hydrocarbons from diesel increased the toxicity, further reducing the growth of both stems and roots[15].

4. Conclusion

This research demonstrated how an auxin-like herbicide (2,4-D) reduced the water content in mesquite seedlings, which hindered their establishment and ultimately resulted in their death during the early stages.

5. References

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